Stergiopoulos Lab

A.   International Peer-Reviewed Journals

† equal first authors; * corresponding author

 

2018

36. Hurlburt NK, Chen LH, Stergiopoulos I*…, Fisher AJ* (2018). Structure of the Cladosporium fulvum Avr4 effector in complex with (GlcNAc) 6 reveals the ligand-binding mechanism and uncouples its intrinsic function from recognition by the Cf-4 resistance protein. PLoS Pathogens. 14(8): e1007263

 

35. Diaz-Trujillo D†, Chong P†, Stergiopoulos I†, Cordovez V, Guzman M, De Wit PJGM, Meijer HJG, Arango Isaza RE, Scalliet G, Sierotzki H, Peralta EL, Kema GHJ* (2018). A new mechanism for reduced sensitivity to demethylation-inhibitor fungicides in the fungal banana black Sigatoka pathogen Pseudocercospora fijiensis. Molecular Plant Pathology, 19(6):1491-1503.

 

34. Onyilo F, Tusiime G, Tripathi JN, Chen LH, Falk B, Stergiopoulos I, Tushemereirwe W, Kubiriba J, Tripathi L* (2018). Silencing of the mitogen-activated protein kinases (MAPK) Fus3 and Slt2 in Pseudocercospora fijiensis reduces growth and virulence on host plants. Frontiers in Plant Science. 9: 291

 

2017

33.  Onyilo F, Tusiime G, Chen LH, Falk B, Stergiopoulos I, Tripathi JN, Tushemereirwe W, Kubiriba J, Changa C, Tripathi L* (2017). Agrobacterium tumefaciens-mediated transformation of Pseudocercospora fijiensis to determine the rRole of PfHog1 in osmotic stress regulation and virulence modulation. Frontiers in Microbiology, 8: 830.

 

2016

32.  Kohler A, Chen LH, Hurlburt N, Salvucci A, Schwessinger B, Fisher A, and Stergiopoulos I* (2016). Structural analysis of an Avr4 effector ortholog offers insight into chitin-binding and recognition by the Cf-4 receptor. The Plant Cell, 28: 1945-1965.

 

31.  Toruño TY, Stergiopoulos I and Coaker GL (2016). Plant pathogen effectors: cellular probes interfering with plant defenses in spatial and temporal manners. Annual Review of Phytopathology, 54:18.1-18.23.

 

30.  Chang TC, Salvucci A, Crous PW and Stergiopoulos I* (2016). Comparative genomics of the Sigatoka disease complex on banana suggests a link between parallel evolutionary changes in Pseudocercospora fijiensis and Pseudocercospora eumusae and increased virulence on the banana host. PLoS Genetics, 12(8):e1005904

 

29.  Arango Isaza RE, Diaz-Trujillo C, Dhillon B, Aerts A, Carlier J, Crane CF, de Jong TV, de Vries I, Dietrich R, Farmer AD, Fereira GF, Garcia S, Guzmán M, Hamelin RC, Lindquist EA, Mehrabi R, Quirós O, Schmutz J, Shapiro H, Reynolds E, Scalliet G, Souza Jr M, Stergiopoulos I, Van der Lee TAJ, De Wit PJGM, Zapater M-F, Zwiers L-H, Grigoriev IV, Goodwin SB, & Kema GHJ* (2016); Combating a global threat to a clonal crop: banana black Sigatoka pathogen Pseudocercospora fijiensis (synonym Mycosphaerella fijiensis) genomes reveal clues for disease control. PLoS Genetics, 12(8):e1005876

 

       Perspective on Manuscripts #30 and #29:

       Friesen TL (2016). Combating the Sigatoka Disease Complex on Banana. PLoS Genetics, e1006234

     

       Selected Media Coverage on Manuscripts #30 and #29:

       Banana disease could threaten global yields (CCTV NEWS)

       Genome sequencing may help avert banana armageddon (University of California)

        UC Davis study finds genome sequencing could avert banana armageddon (Daily Democrat)

       Sequencing of fungal disease genomes may help prevent banana armageddon (ScienceDialy)

       Sequencing of fungal disease genomes may help prevent banana armageddon  (EurekAlert)

       Genomic Studies Tackle Banana Plant Pathogens (Genome web)

       One super-susceptible clone, a versatile fungus and failing fungicides – black Sigatoka and banana (PLoS Blogs)

        Genome Sequencing May Help Avert Banana Armageddon (Technology Org.)

 

28.  Salvucci A, Aegerter B, Miyao GE and Stergiopoulos I* (2016)…; First report of powdery mildew caused by Oidium lycopersici in field-grown tomatoes in California. Plant Disease, 100(7):1497.

 

27.  Mesarich CH†*, Stergiopoulos I†*, Beenen HG, Cordovez V, Guo Y, Jashni MK, Bradshaw RE, and  de Wit PJGM* (2016). A conserved proline residue in Dothideomycete Avr4 effector proteins is required to trigger a Cf-4‒dependent hypersensitive response. Molecular Plant Pathology, 17(1):84-95

 

 

2015

26.  Chang TC and Stergiopoulos I* (2015). Evolutionary analysis of the global landscape of protein domain types and domain architectures associated with family 14 carbohydrate-binding modules. FEBS Letters, 589 (15), 1813-1818.

 

25.  Ben M'Barek S, Cordewener J, Ghaffary SMT, van der Lee T, Liu Z, Gohari AM, Mehrabi R, America A, Friesen TL, Hamza S, Stergiopoulos I, de Wit PJGM and Kema GHJ (2015). FPLC and liquid-chromatography mass spectrometry identify candidate necrosis-inducing proteins from culture filtrates of the fungal wheat pathogen Zymoseptoria tritici. Fungal Genetics and Biology, 79:54-62

 

24.   Chang TC and Stergiopoulos I* (2015). Inter- and intra-domain horizontal gene transfer, gain-loss asymmetry, and positive selection mark the evolutionary history of the CBM14 family. FEBS Jounal, 282(10):2014-2028.

 

23.   Iida Y, Van ‘t Hof P, Beenen H, Kubota M, Stergiopoulos I, Mehrabi R, Notsu A, Fujiwara K, Bahkali A, Abd-Elsalam K, Collemare J, and de Wit PJGM (2015). Novel mutations detected in avirulence genes overcoming tomato Cf resistance genes in isolates of a Japanese population of Cladosporium fulvum. PLoS One, 10(4):e0123271

 

2014

22.   Stergiopoulos I†* and Gordon TR† (2014). Cryptic fungal infections: the hidden agenda of plant pathogens.. Frontiers in Plant Science 5: 506

 

21.   Stergiopoulos I*, Cordovez V, Ökmen B, Beenen H, Kema GHJ and de Wit PJGM* (2014): Positive selection and intragenic recombination contribute to high allelic diversity in effector genes of Mycosphaerella fijiensis, causal agent of the black leaf streak disease of banana. Molecular Plant Pathology 15(5):447–460 

 

2013

20.  Stergiopoulos I*, Collemare J, Mehrabi R and de Wit PJGM* (2013): Phytotoxic secondary metabolites and peptides produced by plant pathogenic Dothideomnycetes. FEMS Microbiology Reviews 37(1):67-93

 

2012

19.  De Wit PJGM, Van der Burgt A, Ökmen B, Stergiopoulos I, Abd-Elsalam K, Aerts AL, Bahkali AHA, Beenen HG, Chettri P, Cox MP, Datema E, de Vries RP, Dhillon B, Ganley AR, Griffiths S, YG, Hamelin RC, Henrissat B, Kabir MS, Karimi-Jashni M, Kema G, Klaubauf S, Lapidus A, Levasseur A, Lindquist E, Mehrabi R, Ohm RA, Owen TJ, Salamov A, Schwelm A, Schijlen E, Sun H, van den Burg HA, van Ham RCHJ, Zhang S, Goodwin SB, Grigoriev IV, Collemare J, Bradshaw RE (2012): The genomes of the fungal plant pathogens Cladosporium fulvum and Dothistroma septosporum reveal adaptation to different hosts and lifestyles but also signatures of common ancestry. PLoS Genetics 8(11): e1003088

 

18.  Stergiopoulos I*, Kourmpetis YA, Slot JC, Bakker FT, de Wit PJGM and Rokas A* (2012): In silico characterization and molecular evolutionary analysis of a novel superfamily of fungal effector proteins. Molecular Biology and Evolution 29(11): 3371–3384

 

2011

17.  Goodwin SB, M'barek SB, Dhillon B, Wittenberg AH, Crane CF, Hane JK, Foster AJ, Van der Lee TA, Grimwood J, Aerts A, Antoniw J, Bailey A, Bluhm B, Bowler J, Bristow J, van der Burgt A, Canto-Canché B, Churchill AC, Conde-Ferràez L, Cools HJ, Coutinho PM, Csukai M, Dehal P, De Wit P, Donzelli B, van de Geest HC, van Ham RC, Hammond-Kosack KE, Henrissat B, Kilian A, Kobayashi AK, Koopmann E, Kourmpetis Y, Kuzniar A, Lindquist E, Lombard V, Maliepaard C, Martins N, Mehrabi R, Nap JP, Ponomarenko A, Rudd JJ, Salamov A, Schmutz J, Schouten HJ, Shapiro H, Stergiopoulos I, Torriani SF, Tu H, de Vries RP, Waalwijk C, Ware SB, Wiebenga A, Zwiers LH, Oliver RP, Grigoriev IV, Kema GH. (2011): Finished genome of the fungal wheat pathogen Mycosphaerella graminicola reveals dispensome structure, chromosome plasticity, and stealth pathogenesis. PLoS Genetics 7(6):e1002070

 

16.  Mehrabi R, Bahkali AH, Abd-Elsalam KA, Moslem M, Ben M'Barek S, Gohari AM, Karimi-Jashni M, Stergiopoulos I, Kema GHJ and De Wit PJGM (2011): Horizontal gene and chromosome transfer in plant pathogenic fungi affecting host range. FEMS Microbiology Reviews 35 (3): 542-554

 

2010

15.  Stergiopoulos I, van den Burg HA, Ökmen B, Beenen H, van Liere S, Kema GHJ and De Wit PJGM (2010): Tomato Cf resistance proteins mediate recognition of cognate homologous effectors from fungi pathogenic on dicots and monocots. PNAS: Proceedings of the National Academy of Sciences of USA 107 (16): 7610–7615

 

2009

14.  De Wit PJGM, van den Burg HA, Mehrabi R and Stergiopoulos I (2009): Fungal effector proteins: past, presence and future. Molecular Plant Pathology 10(6): 735–747

 

13.  Stergiopoulos I and De Wit PJGM (2009): Fungal effector proteins. Annual Review of Phytopathology 47:233–63

 

2008

12.  Bolton MD, van Esse HP, Vossen JH, De Jonge R, Stergiopoulos I, Stulemeijer IJE, van den Berg G, Borrás-Hidalgo O, Dekker HL, de Koster CG, De Wit PJGM, Joosten MHAJ and Thomma BPHJ (2008): The novel Cladosporium fulvum lysine motif effector Ecp6 is a virulence factor with orthologues in other fungal species. Molecular Microbiology 69 (1): 119-136

 

2007

11.  Stergiopoulos I, De Kock MJD, Lindhout P and De Wit PJGM (2007): Allelic variation in the effector genes of the tomato pathogen Cladosporium fulvum reveals different modes of adaptive evolution. Molecular Plant-Microbe Interactions 20 (10): 1271-1283

 

10.  Van Esse HP, Bolton MD, Stergiopoulos I, De Wit PJGM and Thomma BPHJ (2007): The chitin-binding Cladosporium fulvum effector protein Avr4 is a genuine virulence factor. Molecular Plant-Microbe Interactions 20 (9): 1092-1101

 

9.  Stergiopoulos I, Groenevald M, Staats M, Lindhout P, Crous PW and De Wit PJGM (2007): Mating-type genes and the genetic structure of a world-wide collection of the tomato pathogen Cladosporium fulvum. Fungal Genetics and Biology 44: 415-429

 

2006

8.  De Waard MA, Andrade AC, Hayashi K, Schoonbeek H-J, Stergiopoulos I and Zwiers L-H (2006): Impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence. Pest Management Science 62: 195-207

 

2003

7.  Stergiopoulos I, Zwiers L-H and De Waard MA (2003): The ABC transporter MgAtr4 is a virulence factor of Mycosphaerella graminicola that affects colonisation of substomatal cavities in wheat leaves. Molecular Plant-Microbe Interactions 16 (8): 689-98

 

6.  Stergiopoulos I, Van Nistelrooy JGM, Kema GHJ and De Waard MA (2003): Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola. Pest Management Science 59 (12): 1333-43

 

5.  Zwiers L-H, Stergiopoulos I, Gielkens MMC, Goodall SD and De Waard MA (2003): ABC transporters of the wheat pathogen Mycosphaerella graminicola function as protectants against biotic and xenobiotic toxic compounds. Molecular Genetics and Genomics 269 (4): 499-507

 

2002

4.  Stergiopoulos I, Zwiers L-H and De Waard MA (2002): Secretion of natural and synthetic toxic compounds from filamentous fungi by membrane transporters of the ATP-binding cassette and major facilitator superfamily. European Journal of Plant Pathology 108 (7): 719-734

 

3.  Stergiopoulos I, Gielkens MMC, Goodall SD, Venema K and De Waard MA (2002): Molecular cloning and characterisation of three new ATP-binding cassette transporter genes from the wheat pathogen Mycosphaerella graminicola. Gene 289 (1-2): 141-149

 

2.  Stergiopoulos I and De Waard MA (2002): Activity of azole fungicides and ABC transporter modulators on Mycosphaerella graminicola. Journal of Phytopathology 150 (6): 313-320

 

1.  Zwiers L-H, Stergiopoulos I, Van Nistelrooy JGM and De Waard MA (2002): ABC transporters and azole susceptibility in laboratory strains of the wheat pathogen Mycosphaerella graminicola. Antimicrobials Agents and Chemotherapy 46 (12): 3900-3906

 

 

B.   Patents

1.  Stergiopoulos I, Kema GHJ and De Wit PJGM (2009): Method for increasing the resistance of a plant or a part thereof to a pathogen, method for screening the resistance of a plant or part thereof to a pathogen, and use thereof. Patent no: P88514EP00

 

 

C.   Chapters in Books and Full Conference Proceedings

7.  Chong P, Arango R, Stergiopoulos I, Guzmán M, Crous PW, da Silva GF, De Wit PJGM, Kema GHJ (2010): Analysis of azole fungicide resistance in Mycosphaerella fijiensis, causal agent of black Sigatoka. In: Modern Fungicides and Antifungal Compounds VI - ISBN: 978-3-941261-10-5.

 

6.  De Wit PJGM, Joosten MHA, Thomma BPHJ and Stergiopoulos I (2009): Gene for gene models and beyond: the Cladosporium fulvum-tomato pathosystem. In: Plant Relationships / Deising, HB - Berlin Heidelberg: Springer, 2009, The Mycota V - ISBN 978-3-540-87406-5

 

5.  De Wit PJGM, Stergiopoulos I, Van’t Klooster JW, Van Esse PH, Fradin, EF, Ellendorf U, Gabriels SH, Stulemeijer IJ, Vossen JH, Bolton MD, Borras-Hidalgo B, Tameling WT, Abd-El-Haliem AM, Van den Berg-Velthuis GC, Vervoort J, Boeren S, Joosten MHAJ and Thomma BPHJ (2008): Effectors, their targets and host responses in the pathosystem Cladosporium fulvum-tomato. In: Biology of Plant-Microbe Interactions CD/ Lorito, M, Woo, SL, Scala, F, - St. Paul, MN, USA: International Society for Molecular Plant-Microbe Interactions, 2008. Biology of Plant-Microbe Interactions 6 - ISBN: 978-0-9654625-5-6

 

4.  De Wit PJGM, Brandwagt BF, Bolton MD, Van den Burg HA, Van Esse HP, Fradin EF, Gabriëls SHEJ, Ellendorff U, Van der Hoorn RAL, Jones JDG, Joosten MHAJ, Van ‘t Klooster JW, De Kock MJD, Kruijt M, Lindhout PWH, Rooney HCE, Stergiopoulos I, Stulemeijer IJE, Vervoort JJM, Vossen JH, Thomma BPHJ (2006): Functional analysis of Avr2 and Avr4 proteins of Cladosporium fulvum. In: Biology of Plant-Microbe Interactions 5 - ISBN 0965462544

 

3.  Zwiers LH, Stergiopoulos I, De Waard MA (2003): ABC transporters of Mycosphaerella graminicola involved in pathogenesis and multidrug resistance. In: Global Insights into the Septoria and Stagonospora Diseases of Cereals - ISBN 9973-9794-00

 

2.  Stergiopoulos, I (2003): The role of ATP-binding cassette (ABC) transporters in pathogenesis and multidrug resistance of the wheat pathogen Mycosphaerella graminicola. Dissertation Wageningen Universiteit - ISBN 90-5808-787-5

 

1.  Stergiopoulos I, Zwiers LH Gielkens MMC, Goodall, SD, Venema K, De Waard MA (2002): Functional analysis of transporters in the wheat pathogen Mycosphaerella graminicola. In: Modern Fungicides and Antifungal Compounds III - ISBN 3-7862-0144-7

 

 

D.   Publications in Popular Science Journals and Online News Sites

8.  Stergiopoulos I, Drenth A, Kema GHJ (2016): Les bananes vont-elles disparaître ? The Conversation (French Edition)

 

7.  Stergiopoulos I, Drenth A, Kema GHJ (2016): With the familiar Cavendish banana in danger, can science help it survive? The Conversation (US Edition)

 

6. Stergiopoulos I (2016): Genome Sequencing May Help Avert Banana Armageddon. Food and Agriculture News, University of California Davis.

 

5.  Stergiopoulos I, van den Burg HA, Ökmen B, Beenen HG, Kema GHJ,  de Wit PJGM (2010): Homologues of Cladosporium fulvum effector proteins are present in species of Dothideomycetes, are recognized by cognate Cf tomato. Gewasbescherming 41.3 (2010): 149

 

4.  Ökmen B, DE Hollander M,  Stergiopoulos I, van den Burg Ha, de Wit PJGM (2010): Functional Analysis of Cladosponum fulvum Effector Catalog. Gewasbescherming 41(3). - ISSN 0166-6495 - p. 149 - 150

 

 

3.  De Wit PJGM, Bolton M, Boras O, Gabriëls S, van’t Klooster J, Stulemeijer I, Vossen J, van Esse P, Fradin E, Ellendorff U, Stergiopoulos I, Joosten M, Thomma B (2006): Innate immunity of plants against fungi: arms race or balancing selection. Nederlands Tijdschrift

 

2.  Stergiopoulos I, Zwiers LH and De Waard MA (2003): Pompen of verzuipen. Gewasbescherming 34 - ISSN 0166-6495

 

1.  Stergiopoulos I (2003): ATP-binding cassette (ABC) transporters in the wheat pathogen Mycosphaerella graminicola. Gewasbescherming 34. - ISSN 0166-6495